Yours Chromatographically, Kaushik Zala

% CHANGE FROM AQUEOUS TO ORGANIC PHASE MUST BE IN FULL INTEGER, IT MAKES YOUR SEPERATION MORE EASIER AND EFFECTIVE

I.E, % CHANGE IN ORGANIC PHASE IS LIKE 5% ORGANIC CHANGE IN 1 MINUTE

SO, ARRANGE YOUR GRADIENT METHOD JUST LIKE THAT AND SEE EFFECT IN SEPARATION.................................................

FOR MORE METHODS JUST KEEP ME TRACKING..................

WAITING FOR OTHER HPLC SCIENTISTS RESPONSES..........................