Sunday, July 15, 2012
No Peaks or view Small Peaks in HPLC
What You Expect What You got
Disease
|
Medicine
|
Detector off
|
Check Detector
|
Broken connections to recorder
|
Check connections
|
No flow
|
Check flow
|
No sample/Wrong sample
|
Check sample. Be sure it is not deteriorated. Check for bubbles in the vials
|
Wrong settings on recorder or detector
|
Check attenuation. Check gain
|
Disease
|
Medicine
|
Pump off
|
Start pump
|
Flow interrupted
|
Check reservoirs. Check position of the inlet tubing
Check loop for obstruction or air Check degasing of mobile phase Check compatibility of the mobile phase components |
Leak
|
Check fittings
Check pump for leaks and precipitates Check pump seals |
Air trapped in the system
|
Disconnect column and prime pump
Flush system with 100% methanol or isopropanol Contact servicing if necessary |
