Wednesday, February 13, 2019
| Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. The expression HILIC (Hydrophilic Interaction Chromatography) was firstly published by Andrew Alpert in 1990 – since then it took quite some efforts to develop robust and reproducible hydrophilic HPLC phases for HILIC chromatography [A. Alpert, J. Chromatography 499 (1990), 177–196]. HILIC combines the characteristics of the 3 major methods in liquid chromatography – reversed phase (RPC), normal phase (NPC) and ion chromatography (IC): | |||||||
 | Stationary phases (adsorbents) are mostly polar modifications of silica or polymers (SiOH, Amino, Diol, (zwitter) ions, …) – like in NPC. | ||||||
| Mobile phases (eluents) are mixtures of aqueous buffer systems and organic modifiers like acetonitrile or methanol – like in RPC. | ||||||
| Fields of application include quite polar compounds as well as organic and inorganic ions – like in IC. HILIC is NP chromatography of polar and ionic compounds under RP conditions.” 
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